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Biocare Medical dc2002 decloaking chamber
Dc2002 Decloaking Chamber, supplied by Biocare Medical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dc2002+decloaking+chamber/dc2002+decloaking+chamber/pmc11460503-302-14-12
Average 90 stars, based on 1 article reviews
dc2002 decloaking chamber - by Bioz Stars, 2026-09
90/100 stars

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Stripping Membranes:

Article Title: Assessment of patient-to-patient and intra-individual human abdominal skin immune cell variability
Article Snippet: Four-micrometre-thick sections were dewaxed and rehydrated using a Leica Autostainer XL (Leica Microsystems Pty Ltd., Mt Waverley, Australia) and incubated in 0.5% H 2 O 2 in Tris-buffered saline for 5 min to quench endogenous peroxidase. .. Antigen retrieval and antibody stripping was performed using a Biocare Medical DC2002 Decloaking Chamber (Biocare Medical, Pacheco, USA). .. Primary antibodies (from Dako and Abcam; anti-FoxP3 [clone 22510); anti-CD4 (clone 4B12), anti-CD3 (polyclonal), anti-HLA-DR (clone TAL.1B5), anti-neutrophil elastase (clone EPR7479), anti-CD14 (clone EPR3653), anti-CD1a (clone EP3622), anti-Langerin (clone EPR15863), anti-CD11c (clone EP1347Y), anti-CD69 (clone EPR21814), and anti-CD8 (clone C8/144B)) were incubated at room temperature for 30 min.

Staining:

Article Title: The KDM5A/RBP2 histone demethylase represses NOTCH signaling to sustain neuroendocrine differentiation and promote small cell lung cancer tumorigenesis
Article Snippet: Slides were baked in an Isotemp Oven (Fisher Scientific, Pittsburgh, PA) at 60°C for 30 min before staining. .. For antigen retrieval, slides were heated with a DC2002 Decloaking Chamber (Biocare Medical, Pacheco, CA) to 125°C for 30 sec and 90°C for 10 sec in EDTA buffer (pH = 8, Thermo Scientific cat. no. Ap-9004-500) for synaptophysin staining, or citrate (pH = 6.0, Thermo Scientific cat. no. Ap-9003-500) for Chromogranin A, Ascl1, Notch1, Notch2, and H3K4me3 staining. .. All sections were incubated with peroxidase (Dako, Carpinteria, CA) and protein (Dako) blocking reagents for 5 min each.

Article Title: Regulation of neuroendocrine plasticity by the RNA-binding protein ZFP36L1
Article Snippet: Slides were baked in an Isotemp Oven (Fisher Scientific) for 30 min at 60 °C to melt excess paraffin. .. For antigen retrieval, slides were heated with a DC2002 Decloaking Chamber (Biocare Medical, Pacheco, CA) to 125 °C for 30 s and 90 °C for 10 s in citrate (pH = 6.0, Thermo Scientific cat. no. Ap-9003-500) for ASCL1 and ZFP36L1 staining. .. All sections were incubated with peroxidase (Dako cat. no. S2003, Carpinteria, CA) and protein (Dako cat. no. X0909) blocking reagents for 5 min each.

Article Title: Prion-induced photoreceptor degeneration begins with misfolded prion protein accumulation in cones at two distinct sites: cilia and ribbon synapses
Article Snippet: The secondary antibody was biotinylated goat anti-human IgG at 1:500 dilution (Jackson ImmunoResearch, West Grove, PA.), and avidin-horseradish peroxidase was used with DAB as chromogen (DAB Map kit; Ventana Medical Systems, Tucson, AZ.). .. For immunofluorescent staining, antigen retrieval for all targets was performed using a Biocare Medical DC2002 Decloaking chamber with sodium citrate buffer at pH 6.0(0.01 M) for 20 min at 120° C / 20 PSI and cooled to 50 °C. ..

Article Title: The prion protein is required for normal responses to light stimuli by photoreceptors and bipolar cells
Article Snippet: The secondary antibody was biotinylated goat anti-human IgG at 1:500 dilution (Jackson ImmunoResearch, West Grove, PA.), and avidin-horseradish peroxidase was used with DAB as chromogen (DAB Map kit; Ventana Medical Systems, Tucson, AZ.). .. For immunofluorescent staining, antigen retrieval for all targets was performed using a BioCare Medical DC2002 Decloaking chamber with sodium citrate buffer at pH 6.0(0.01 M) for 20 min at 120°C/20 PSI and cooled to 50°C. ..

Article Title: Prion-induced photoreceptor degeneration begins with misfolded prion protein accumulation in cones at two distinct sites: cilia and ribbon synapses.
Article Snippet: The secondary antibody was biotinylated goat anti-human IgG at 1:500 dilution (Jackson ImmunoResearch, West Grove, PA.), and avidin-horseradish peroxidase was used with DAB as chromogen (DAB Map kit; Ventana Medical Systems, Tucson, AZ.). .. For immunofluorescent staining, antigen retrieval for all targets was performed using a Biocare Medical DC2002 Decloaking chamber with sodium citrate buffer at pH 6.0(0.01 M) for 20 min at 120° C / 20 PSI and cooled to 50 °C. ..

Article Title: Regulation of Neuroendocrine Plasticity by the RNA-Binding Protein ZFP36L1
Article Snippet: Slides were baked in an Isotemp Oven (Fisher Scientific) for 30 minutes at 60°C to melt excess paraffin. .. For antigen retrieval, slides were heated with a DC2002 Decloaking Chamber (Biocare Medical, Pacheco, CA) to 125°C for 30 seconds and 90°C for 10 seconds in citrate (pH = 6.0, Thermo Scientific cat. no. Ap-9003-500) for Ascl1 and Zfp36l1 staining. .. All sections were incubated with peroxidase (Dako cat. no. S2003, Carpinteria, CA) and protein (Dako cat. no. X0909) blocking reagents for 5 minutes each.

Article Title: Regulation of neuroendocrine plasticity by the RNA-binding protein ZFP36L1.
Article Snippet: Slides were baked in an IsotempOven (Fisher Scientific) for 30min at 60 °C tomelt excess paraffin. .. For antigen retrieval, slides were heated with a DC2002 Decloaking Chamber (Biocare Medical, Pacheco, CA) to 125 °C for 30 s and 90 °C for 10 s in citrate (pH = 6.0, Thermo Scientific cat. no. Ap-9003-500) for ASCL1 and ZFP36L1 staining. .. All sections were incubated with peroxidase (Dako cat. no. S2003, Carpinteria, CA) and protein (Dako cat. no. X0909) blocking reagents for 5min each.



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